Saturday, 30 May 2015

B.M. Patil and G.M. Rane as Pride of Nation Rose to Pride of World with Landmark Ernest Rutherford Research Award-2015 in Agriculture

Breaking News: B.M. Patil and G.M. Rane as Pride of Nation Rose to Pride of World with Landmark Ernest Rutherford Research Award-2015 in Agriculture

B.M. Patil and G.M. Rane as Pride of Nation Rose to Pride of World with Landmark Ernest Rutherford Research Award-2015 in Agriculture

30 May, 2015, India, USA NEWS CORP

Cluster bean, gamma rays, mutagen, genetic variability, mutagenesis, KR: Kilo Rongten, R.B.D.: Randomized Block Design, EMS: Ethyl Methane Sulphonate, S.A.: Sodium Azide, Gy: Grays, International Journal of Agriculture, Photon Journal,  Photon Foundation,, HS Result 2015, Bajrangi Bhaijaan, San Andreas, Premam, Mass, Google Photos, Welcome to Karachi review, FIFA elections 2015, Sikh boy viral video, Gujjar reservation, Masaan India release, Lorry rental, French Open 2015, UEFA boycott FIFA, Mysore new King, Oxford female vice-chancellor, Dharmendra hospitalCluster bean, gamma rays, mutagen, genetic variability, mutagenesis, KR: Kilo Rongten, R.B.D.: Randomized Block Design, EMS: Ethyl Methane Sulphonate, S.A.: Sodium Azide, Gy: Grays, International Journal of Agriculture, Photon Journal,  Photon Foundation,, HS Result 2015, Bajrangi Bhaijaan, San Andreas, Premam, Mass, Google Photos, Welcome to Karachi review, FIFA elections 2015, Sikh boy viral video, Gujjar reservation, Masaan India release, Lorry rental, French Open 2015, UEFA boycott FIFA, Mysore new King, Oxford female vice-chancellor, Dharmendra hospital

B.M. Patil and G.M. Rane, given the rock star fame in Agriculture, as declared among World’s 500 Most Influential Agronomists for Year 2015 on Earth. In a worldwide meritorious competition, B.M. Patil and G.M. Rane grabbed the world’s prestigious Ernest Rutherford Research Award-2015 in Agriculture. Representing the great legacy of India, B.M. Patil and G.M. Rane established the matter of proud to civilians of India. International Media is now seriously looking towards India, since B.M. Patil and G.M. Rane made a remarkable historical achievement. Agronomists are ensured that under the leadership of B.M. Patil and G.M. Rane, millions of emerging intellectuals would be benefited and inspired around the world.

Prof. Patil Balasaheb Madhukar is Asst.Prof in Botany at Department of Botany, J.A.T. Arts Science & Commerce, College (For Women), Malegaon, Dist. Nashik, Maharashtra, India. He is affiliated with University of Pune, Maharashtra, India. He has completed his M.Sc and B.Ed. His areas of Research are Cytogenetics & Plant Breeding. He has deep interest in Genetics, Cytogenetics, Molecular Biology & Biotechnology. His current Research is going on Ph.D in Botany. He received Ideal Teacher Award 2006, Best Research Paper Presentation Award on National Level RAAB 2012 and Best Research Paper Presentation award in National level AMBI-2014.

Dr. Mrs. Gauri Milind Rane is Associate Professor & Head at P. G. Research Centre, Department of Botany, & Coordinator, JalaSRI, Watershed Surveillance & Research Institute, M. J. College, Jalgaon, India. He has completed M.Sc and Ph.D from University of Pune. He has received Certificate in Applications of Remote Sensing & GIS in Forestry & Ecology from Indian Institute of Remote Sensing, Dehradun, India.

B.M. Patil and G.M. Rane from India made a historical victory over the international competition and won Ernest Rutherford Research Award-2015 in Agriculture. Their research was highlighted in international media and gathered a large worldwide attention in scientific circles while it appeared in world's most read journal 'International Journal of Agriculture'. In their report on Effect of Gamma Rays on Germination, Pollen Sterility and Plant Survival in Cluster Bean they mention that The seeds of cluster bean (Cyamopsis tetragonoloba (L) Taub) Variety NCB.12/ Nandinee were irradiated with Gamma rays at different doses to determine their effect on seed germination, seedling height, pollen sterility and plant survival at maturity in M1 generation. Gamma rays are the most widely used physical mutagen in crop improvement. Gamma rays are electromagnetic radiations with the highest energy level. They are well known for their action in causing extensive damage to DNA molecules and producing strand breaks and destruction of sugars and bases. In the present investigation genetically pure, dry, and non dormant seeds of cluster bean (2n=14) of cultivar NCB-12 were irradiated with physical mutagen gamma rays using 60CO as a source of radiation at of doses 10 kR, 20 kR, 30 kR, 40 kR and 50 kR. The seeds were sown immediately in the field to raise the M1 generation with three replications in a Randomized Block Design. Different biological parameters like seed germination, seedling height, pollen sterility and plant survival at maturity were recorded in M1 generation. It was concluded that the percent seed germination, seedling height and plant survival at maturity decreased with increasing doses and gamma rays but the pollen sterility increased with increasing doses of gamma radiation. The maximum germination percentage was 94% in 10KR and least 65% in 50 KR dose. The seedling height ranged from 14.3 to 7.69 cm from 10 kR to 50 kR doses. The maximum pollen sterility was 32.50% in 50 kR dose and least in 10 kR dose. The percent plant survival at maturity was highest 83.90% in 10 kR and lowest 68.72% in 50 kR dose of gamma rays. The reduction of these parameters was prominent due to the treatment of gamma rays. Under their leadership the research area is surely likely to expand beyond the horizons. It will be worthy to explore the further potential under their legacy. B.M. Patil and G.M. Rane as valuable intellectuals for the India offer exclusive opportunities for scholars around the world. One of the most attractive weightage for India is to have the awardees for promotion of the concerned scientific discipline for the benefit of its civilians. Thus India which has excellent track record to produce brain storming intellectuals, has offered a new extension in terms of B.M. Patil and G.M. Rane to world.

In present investigation, it has been observed that percent seed germination, seedling height and survival of plants at maturity have decreased with an increased dose of gamma rays. Thus there is an inverse relation between dose of mutagen and percent seed germination, seedling height and plant survival at maturity. The observed reduction in percent seed germination, seedling height and plant survival at maturity in M1 progeny might be due to conversion of certain functional genes into non functional ones, due to the action of mutagen gamma rays.

The pollen sterility increased with increased doses of gamma rays. The pollen sterility was highest at higher doses of gamma rays showed its high sensitivity with increasing doses of gamma rays. This induced pollen sterility in cluster bean may be as a result of chromosomal aberrations especially high frequency of translocations.

Physical mutagen gamma rays showed an inhibitory effect on percent seed germination, seedling height and plant survival at maturity but promontory effect on pollen sterility in M1 generation.

Thus in the present investigation the doses of gamma rays used in cluster bean Var. NCB-12 will be effective in induction of wide range of mutants may create useful genetic variability for crop improvement.

In recent years, induction of mutations employing mutagens has widely been accepted as an excellent tool for creating genetic variability and as supplementary approach in crop improvement programmes in number of plants. In the present investigation a physical mutagen gamma rays has been used for induction of mutations in cluster bean Var. NCB-12.

In the present investigation, emphasis was given to record the effect of 10, 20, 30, 40 and 50 kR doses of gamma rays on biological parameters like seed germination, seedling height, pollen sterility and plant survival at maturity, in M1 generation of cluster bean variety NCB-12.

The altered frequencies of these parameters in M1 generation as compared to control revealed the sensitivity of mutagen gamma rays in cluster bean. These biological parameters are useful in understanding the effectiveness and efficiency of mutagen used further in M2 and M3 generations to create the genetic variability in the crop.

This investigation will also help in genetic improvement and creation of novel varieties of cluster bean containing high yielding, disease resistant and early maturing verities.

In the present investigations, M1 progeny were analyzed for parameters like percent seed germination, seedling height, pollen sterility and plant survival at maturity. Altered frequencies of all these parameters in M1 generation as compared to control results in the biological damage. During this work increased male sterility was one of the limitations in the self pollinated crop like cluster bean. Further in the mutagenic induced generations like M2 & M3 raised from M1, the identification of micro mutations, which are more useful would be very difficult because the useful mutations may produced at very low frequency. These induced mutations might be deleterious and undesirable. Desirable mutations were commonly associated with undesirable side effects due to other mutations, chromosomal aberrations, sterility etc. These limitations may create the barriers in induction of genetic variability for crop improvement in cluster bean.

The out crossing with other varieties was avoided in the M1 generation, since M1 plants shows considerable male sterility. The prevention of out crossing was necessary in the self pollinated crop like cluster bean and this was done by isolation. The undesirable mutations appeared in M2 or M3 generations would be removed by back crossing the mutants with the parent variety. The frequency of useful desirable mutations is very low hence a very large population of cluster bean would be grown in M2 and subsequent generations and carefully studied to identify and isolate desirable mutants for crop improvement in cluster bean.
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